ags human gc cell line  (ATCC)

Journal: STAR Protocols

Article Title: Protocol for isolating patient-derived ascites cells and extracellular vesicles from gastric cancer peritoneal metastases

doi: 10.1016/j.xpro.2025.104133

Figure Lengend Snippet: NTA of exosomes derived from patient ascites and AGS gastric cancer cells Representative NTA profiles showing the size distribution and particle concentration of exosomes isolated from (left) patient-derived ascites and (right) AGS cell-conditioned media. Both populations exhibit a typical exosome size range of approximately 30–150 nm.

Article Snippet: Human: AGS cell line , ATCC , N/A.

Techniques: Derivative Assay, Concentration Assay, Isolation

Journal: iScience

Article Title: Depletion of LINC01133 from cancer-associated fibroblasts exacerbates the progression of gastric adenocarcinoma

doi: 10.1016/j.isci.2025.113886

Figure Lengend Snippet: Working model of CAF-derived LINC01133 depletion boosts gastric adenocarcinoma progression

Article Snippet: The human AGS gastric adenocarcinoma cell (GAC) line was obtained from ATCC.

Techniques: Derivative Assay

Journal: Signal Transduction and Targeted Therapy

Article Title: Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses

doi: 10.1038/s41392-025-02523-1

Figure Lengend Snippet: Selective and efficient depletion of SAMHD1 in various tumor types following HSP90 inhibition. a siRNA-mediated knockdown of HSP90 in Molm-13 cells reduced SAMHD1 protein abundance ( n = 3). b Quantification of SAMHD1 and HSP90 protein levels, normalized to GAPDH; Data are presented as mean ± s.d. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). c HEK 293T, AGS, H9, HGC-27, HeLa and d Inoculation of THP-1 cells into 12-well cell culture plates and treated with indicated doses of Pimitespib, IPI-504, PU-H71, or STA-9090 for 18 h. SAMHD1 protein levels were analyzed by western blot ( n = 3). e Protein quantification results from the experiments in ( d ) ( n = 3). f Western blot assay of SAMHD1 protein levels in primary AML blasts derived from patients, following exposure to different concentrations of HSP90 inhibitors for 18 h ( n = 2 patient samples). g RT-qPCR analysis of SAMHD1 mRNA levels in THP-1 cells treated with indicated concentrations of HSP90 inhibitors (IPI-504, PU-H71, or STA-9090) for 18 h ( n = 3), with GAPDH as a normalization control. Data are presented as mean ± s.d. h Cycloheximide (CHX) chase analysis of SAMHD1 protein stability in THP-1 cells. Cells were treated with 25 μM CHX, with or without 1 μM STA-9090, and harvested at the predefined time points ( n = 3). i Time course analysis of SAMHD1 protein in THP-1 cells. Cells were treated with 200 nM IPI-504, 200 nM PU-H71, or 20 nM STA-9090 and harvested at the indicated time points for western blot analysis. j MTT assay measuring THP-1 cell viability after 18-hour treatment with various doses of HSP90 inhibitors ( n = 3). Data are presented as mean ± s.d. k Diagram illustrating the source organs of the cell types used. l Heatmap displaying SAMHD1 protein abundance in the specified cell lines after 18-hour treatment with 20 nM STA-9090, quantified using ImageJ. GAPDH functioned as a normalization control

Article Snippet: Human hematological malignancy cell lines THP-1, Kasumi-1, Molm-13, and H9; human solid tumor cell lines AGS, HGC-27, HeLa, HEK293T, A549, HCT-29, and HT-116 were purchased from the American Type Culture Collection (ATCC) and cultured in accordance with the ATCC’s recommendations.

Techniques: Inhibition, Knockdown, Quantitative Proteomics, Cell Culture, Western Blot, Derivative Assay, Quantitative RT-PCR, Control, MTT Assay